01 M HCl was added, and absorbance was read at 570 nm using a Spectramax M5 plate reader (Molecular Devices). The IC50 was calculated using the DRC package in the R software program (v3. At predetermined time points, the CCK-8 reagent was added into representative wells and the plate was incubated at 37°C for 1 h. The absorbance at 450 nm was subsequently recorded with SpectraMax M5 plate reader (Molecular Devices). 1 g/ml SDS solution in 0. Then,SureBlue Reserve TMB Peroxidase Substrate (KPL Inc) was used for detection and absorbance at 450 nm was measured on a SpectraMax M5 plate reader (Molecular Devices). Appropriately. Three kits covering a wide range of dsDNA concentrations. BMW M5 Forum and M6 Forums. The solution was shaken at 37°C and 1200 rpm for 3 days. The plastic from a microplate will affect the light polarization, precluding bottom reads and reading a covered plate. For a Price List, please call (858) 646-3100 ext. 12. In a typical experiment 2 μL of freshly prepared 5-nitrobenzisoxazole stock solution in acetonitrile was added to 200 mL of buffered (10–25. Then, luminescence of samples was read in a Spectramax M5 plate reader with integration time of 5 s. LbL films are immersed into 20 mM DTT in PBS buffer (2. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode Detection Platform. 2% acetamide. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. GloMax® Discover is a ready-to-use multimode plate reader developed with Promega reagent chemistries to provide a simple means of detecting luminescence, fluorescence and absorbance. 5. Fluorescence values were used to calculate the percent inhibition of enzyme activity relative to maximal activity, PTP4A3 in the absence of inhibitor, and maximal inhibition, PTP4A3 in the presence of 2 mM Na 3 VO 4. We therefore gathered salient. edu The content of this site is published by the site owner(s) and is not a statement of advice, opinion, or information pertaining to The Ohio State University. 4. 0. The LMax plate reader has similar calculations and its RLUs are comparable to those of LMax II 384. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. 3. 3465 or email us. Ensure data accuracy of your reader. GraphPad Prism was used to process the data and calculate the Km and Vmax values. How-to-use guide for the M5 plate reader and SpectraMax Software in the CMB. Options include top and bottom detection, temperature control and reagent injector. We would like to show you a description here but the site won’t allow us. SpectraMax Paradigm Multi-Mode Safety Guide fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). The SpectraMax 190 reader can be integrated with Molecular . 6 for further experiment, so I'm confused which i would choose for OD value determination. 0 . Purified fluorescent protein was diluted 15-fold into 0. To quantify the cell apoptotic degree, the harvested cells were stained with. PMC full text: Oncotarget. Detection cartridge modularity allows for easy addition of new read modes within minutes without. T o enable accurate quantita-tion, initially standard curves were plotted f or both Pi and. 25 fmol/well, 384-well plate)−top Fluorescein 4 pM (0. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). Lastly, Jurkat cells were least susceptible to Vorinostat treatment (all IC 50 > 50. Human T-cells were cultured at 1 × 10 6 /mL for 5 days with plate-bound 10 μg/mL anti-CD3 and soluble 1 μg/mL anti-CD28. Plate chamber design of the SpectraMax category 1 microplate readers. 2. Therefore you need to ask a physicist. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. 4. Page 1 of 8 . Thermo Scientific Microplate Reader Verification Plates are dedicated performance verification systems helping to verify and document the key performance parameters of Thermo Scientific Multiskan FC, Multiskan Sky, Luminoskan, Fluoroskan FL, and Varioskan LUX. The patented Hybrid Technology offers flexibility and sensitivity across a broad range of applications in the clinical laboratory. Endothelial permeability was also measured in vitro, using a transwell assay. Imagine a world where everyone smiles®. There is currently no charge for using this plate reader. 1) Select the correct plate and the wells that you want to measure. The purple holder is used for reading non-transparent plates from the top (e. A microplate reader with fluorescence intensity (FI) detection uses a light source, usually a Xenon flash lamp or LED, to excite a fluorophore (fluorescent molecule) at a particular wavelength. All kinetic reads were taken at 25 °C in nonbinding all black clear-bottomed Greiner 96-well plates covered with optically clear films and stirred for 10 s prior to each reading. 3) At the bottom of the strip, click on "Show. After mixing all the components, the absorbance of the mixture was continuously measured at 475 nm at 25°C using a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA, USA). 1 and slit widths of 2. Detection modalities. Following lysis, the plate was centrifuged at 500g for 3 min and supernatants transferred to a Maxisorp 96-Well Flat-Bottom Assay Plate (Nunc). Abs). 8 cm x 41. Specifications General -Detection mode Fluorescence, Luminescence -Read method End point, kinetic. The absorbance was measured using Molecular Devices Spectramax M5 plate reader with the software Softmaxpro at 570 and 650 nm. After 24 h, cells were treated as described for the individual figures, then assays were performed 48 h after transfection. Typical settings for fluorescein are 485/520 and. Cells were seeded at a density of 1,000 cells/well (A549: 500 cells/well). Select Update Driver. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. 5 hours after an initial 10-minute incubation period. The spectrophotometric performance of the SpectraMax M2 and SpectraMax M2 e is similar to the SpectraMax Plus, a dedicated absorbance plate reader. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Fe standards require only about 30 minutes but can be kept in 1x working solution for at least a week withoutAfter 15 min, the absorbance at 550 nm was recorded using a Molecular Devices SpectraMax M5 plate reader. Vials were coded so that the person running the samples was blinded to the contents. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. Kinetic data for Omnia® assays on the SpectraMax® M5/M5e reader. HUVECs were seeded in 12-well culture plates at 5 × 10 4 cells/well, and incubated for 24 h. The drawing shows a standard microplate of 15 mm height covered with a lid. Data were expressed as % control =. 7°C (gradient heating design). 1. Thus, the. Read More. Fluorescence Microplate Reader Comparison Chart. Enter Sample IDs (HPV-Type, Sample Description and Data Reference when applicable), Dilution Factors, and background information into the template. Application Area: Multi mode plate reader. 5: Schematic of the back panel of a reader. The plates were washed thrice with kit wash buffer; 150 µl kit substrate was then added to each well and the plate incubated 30 min at room temperature (with shaking). 1% Tween-20 and bound secondary was detected using Ultra TMB (Thermo Fisher); signals were read on a Molecular Devices Spectra Max M5. For example, microplates containing different samp les can be read using the same orChoose the desired plate type, using the upper dropdown menu to choose plate format (96 or 384 wells) and the "Select Specific" menu to choose the specific plate type. Standards and QCs are composed of a. Our lab has a BMG labtech Pherastar FS, it is easy to use and we have never had any issue with it. Figure 1. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. Information on cell morphology, localization of signal, cell count, and more is obtained with Cytation 5 imaging mode. Microplate Capacity. Microtiter plates (Corning 3690; 96-well half area plates) were coated overnight at. The plate reader assay was then repeated in SC-URA media with and without. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. 2. BioTek Synergy H1 is a modular multimode microplate reader, with monochromator-based optics and filter-based optics. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Spend a little more and you can get an FTDI-based USB cable. Absorbance was measured at 563 nm using a SpectraMax M5 plate reader (Molecular Devices). the contents were carefully mixed by pipetting up and down. Inhibition assays at multiple compound. The solids content was measured using a MA35 Moisture Analyzer (Sartorius AG). The results were normalized to the amount of total protein compared to the sham. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode…. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. 4: Test tube cover. After 30 min, the plate was washed with PBS 0. Fluorescence, luminescence, and UV-Vis absorbance multimode plate reading. The average 526/470 ratio for the sample aliquots. 1. There is currently no charge for using this plate reader. Download a PDF version of the SpectraTest validation package. The sample is typically placed in a cuvette through which light is sent horizontally. The empty LNP matrix solution was made by mixing 300 μL of empty LNP solution with 45 mL diluent. To update the driver. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the plate setup helper shown at the cursor below: 8. Specifications General -Detection mode Fluorescence, Luminescence -Read method End point, kinetic. Guidelines to using the Qubit Flourimeter. Optimal timing of signal detection occurred 15 min after reaction initiation with the kit substrates. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. Microplate Readers Fisher Scientific AccuSkan FC Microplate Reader – 2020. 2. 0 . The samples were loaded into a rectangular quartz cell with the light path length of 1 mm. Fluorescence, brightfield, color brightfield, and phase contrast imaging are available with the automated cell imager. 6. 9. 2. Absorbance readings were then taken at 650 nm using a Spectramax M5 plate reader with SoftMax Pro v5. The Formazan crystals formed in each well were dissolved by adding 100 µl DMSO. 1. 130 Watts max. Product Type. Chemistry Department Facilities. Description Figure 1. 09 in One Micro Manipulator Reader software. A solution of phenol red-free MEM containing 1. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. HSL_EQ_005: Use and Maintenance of a Molecular Devices M5 Plate Reader in the HPV Serology Laboratory 3. 1 or 10 Tablet PC with USB connection to instrument. coli cells were purchased from New England Biolabs (Ipswitch, MA). The following components are located on the back panel of the SpectraMax and VersaMax readers: • Power switch: Step 4: Incubate at room temperature for 1 hour, protected from light. 9. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. edu to schedule training. M5 SpectraMax M5e Absorbance X X X X Fluorescence intensity X X X X Fluorescence polarization XX Time-resolved fluorescence XX X Luminescence x x X X Note: In this user guide, all references to SpectraMax Multi-Mode Microplate Readers include the M3, M4, M5, and M5e models. Cytation 5 combines automated microscopy and conventional microplate detection in a configurable, upgradable platform. 1. The Back Panel Figure 1. Plate Reader (3) Name Price; EnSpire Flexstation 3 SpectraMax M5 Plate. Most papers they cultured bacteria to OD600>0. After this time period, the plate was read for fluorescence intensity at ex/em wavelengths of 532/590 nm, on a SpectraMax M5 plate reader. Temperature variation ± 0. FeCl 3, iron standards are always included (0-4 µg /ml). a validation plate, certificate of calibration, user guide, and automated SoftMax® Pro Software protocols to run validation tests. 1. Lucifer yellow was measured using an M5 plate reader (Molecular Probes) at an excitation wavelength of 485 nm and an emission wavelength of 530 nm. The luminescent signal was recorded on a SpectraMax M5 plate reader (Molecular Dy-namics). Linear, Orbital Slow, and Orbital Fast. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. a validation plate, certificate of calibration, user guide, and automated SoftMax® Pro Software protocols to run validation tests. Wells with an OD 600[0. 6), turn on the M5 Plate Reader and open the “BCA Template” file 9. Power on the Epoch Biotek Gen 5 absorbance microplate reader. Please contact Tatiana to reserve the plate reader time: [email protected] or . The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. DH5α and BL21 (DE3) chemically competent E. Concentrations of other cytokines were determined by using custom MSD kits and a QuickPlex SQ120 plate reader (Meso Scale Discovery,. 2013): the specific activity of both enzymes was ~1. There is currently no charge for using this plate reader. Fuchs1,2,3* 1 Department of Biology, Tufts University, Medford, MA 02155, USA 2 Institute for Protein Innovation, Boston, MA 02115, USA 3 Program in Cellular and Molecular Medicine,. Reader Documentation Version No. Evans blue dye in the supernatant was detected at optical density (OD) 620 nm, using a SpectraMax M5 plate-reader (Molecular Devices, Sunnyvale CA). 4-liter V-8 that sends power to all four wheels via the car’s. 9000-0756. The same procedure. based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. S. Nonspecific binding was determined by competition with 1. 0 mM of NADH per min at pH 8. 40266 Email: [email protected] fluorescence intensity was determined using a fluorescent plate reader (SpectraMax M5 plate reader) set at an excitation wavelength of 485 nm and an emission wavelength of 530 nm. 5. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). 2. Then the plate was incubated at 37 °C for 2 h. 9. The ROS level was expressed in arbitrary units (DCF mg −1 protein). For example, microplates containing different samp les can be read using the same orThe 526/470 ratios for the standards were plotted against the standard concentrations. Developed with Promega reagents to provide a. 99 was required for a valid standard curve. ThT fluorescence was measured at 444 and. 5. The sample is typically placed in a cuvette through which light is sent horizontally. Incubation. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. Published Dec 16, 2020 | Updated Dec 16, 2020. Use of Attune Flow Cytometer, Leica Cryostat, M5 Plate Reader, and Typhoon 5 require training; please contact Cell_Bio_Operations at hms. com 9. The Chemistry Department is housed in an air-conditioned building, sharing space with the Departments of Biology and Molecular Biology & Biochemistry. 5: Schematic of the back panel of a reader. 600 in a Molecular Devices SpectraMax M5 plate reader at 30˚C with shaking prior to each reading. The data analysis was performed by GraphPad Prism. I’m. The drawing shows a standard microplate of 15 mm height covered with a lid. After 48-h growth at 25 C with agitation, the optical density was recorded using a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA, USA). Supersedes 1. 5. Open and turn on the laptop. SpectraMax M5 Plate Reader (Molecular Devices) If you have trouble accessing this page and need to request an alternate format, contact [email protected] H1 Hybrid Multi-Mode Reader Synergy™ H1 is a configurable multi-mode microplate reader. Readings were taken every 30 minutes. OD 600 and luminescence were measured at 30 minute intervals for 48 hours. 5 pM (0. To evaluate the safety of the formulas against eyes, we used the rats that were topically administrated with one drop (20 μL) of MT, MT-LIPs, 5% TAT-MT-LIPs, or 10% TAT-MT-LIPs (all at the equaled MT-concentration of 100 μM) at a. 9. Turn the reader on and secure the USB cable. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Plates were incubated at 37°C for 6 hours and then equilibrated to room temperature for 30 min. The following document is. The following components are located on the back panel of the SpectraMax and VersaMax readers: • Power switch: based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. The Back Panel Figure 1. 6 for further experiment, so I'm confused which i would choose for OD value determination. A reaction volume of 12 µL was placed into each well of 384-well clear bottom black-walled plate that was sealed with an oxygen impermeable membrane. 7. The Back Panel Figure 1. 2M posts 124. To avoid these issues and provide the best possible dynamic range between the highest and the lowest measurement values of your assay, fluorescence gain is typically adjusted on the sample with the expected highest signal output (e. Shop now. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. Please talk to your your local Tecan representative for the right configuration which suits your application demands. Enter Sample IDs (HPV-Type, Sample Description and Data Reference when applicable), Dilution Factors, and background information into the template. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. ScanLater Western Blot Detection Cartridge. The IC50 was calculated using the DRC package in the R software. plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well microplates. 2. GloMax® Discover is a ready-to-use multimode plate reader developed with Promega reagent chemistries to provide a simple means of detecting luminescence, fluorescence and absorbance. 4. . SpectraMax M5 plate reader (Molecular Devices, San Jose, CA) was used. were read using SpectraMax M5 plate reader (Molecular Devices, San Jose, CA, USA) with excitation of 380 nm and emission of 460 nm. Analyst, M5, Flex 3, and SpectraMax L readers work differently. In the closed conformation, the regulatory SH3 and SH2 domains pack against the back of the kinase domain, providing allosteric control of kinase activity. The EPOCH2 can easily develop various types of assays (wavelength scan: 200–999 nm), and its temperature control (up to 65 c) is excellent. 7°C and the bottom to 36. 14. Product Overview. The SpectraMax iD5 User Guide is a comprehensive document that provides detailed instructions on how to set up, operate, and maintain the SpectraMax iD5 Multi-Mode Microplate Reader. The dual monochromator optics allow. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Uncontrolled Copy for Training and Reference Purposes Only . 1. An emission wavelength of 535 nm was used for carboxy-H2DCFDA and 610 nm was used for DHE and MitoSOX ™. PMC full text: Oncotarget. 6-, 12-, 24-, 48-, 96- and 384-well plates. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. Representative fluorescence images of the cell-containing scaffolds and well plate are shown in Fig. 19 , 25 Human umbilical cord endothelial cells (ATCC, Manassas, VA) were grown in 24-well plates coated with. 5 h (during the lag phase), 7 h (during the exponential phase), and 24 h (during the plateau phase) for analysis of the fermentation. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. The fluorescence was then measured with a fluorescence plate reader (SpectraMax M5; Molecular Devices, Inc. Reactions on 384-well plates (Corning) were monitored by fluorescence (emission 545 nm) using a SpectraMax M5 plate reader (Molecular Devices), with excitation at 274 nm, for 20 min at 2-min. During the room temperature incubation (step 9. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. 7. M-5 The M-5 multitronic unit, or the M-5 computer, was an advanced multitronic computer system and prototype created by Doctor Richard Daystrom during. Turn on the SpectraMax M5 Multi-Mode Microplate Reader. 9. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400–750 nm for readings in fluorescence polarization mode (M5, M5e models only). limit of detection (LLD) for ATP was estimated for each microplate reader using theThe relabeled data may be saved for further analysis as either a . Last lab had ours for years and never had a problem with it. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. Extreme caution is recommended when you use organic solvents. 0200-0729. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microp late Readers by. AB-0558) and immediately read for luminescent signal on a SpectraMax M5 plate reader. 4: Test tube cover. Luminescence was measured on a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA) after the addition of nano-Glo substrate (Promega, Madison, WI) in accordance with the manufacturer’s directions. Protein concentration of viral suspensions was measured using a Pierce BCA Protein Assay Kit (23225, Thermo Fisher Scientific) with a mucin standard curve read on a SpectraMax M5 plate reader (Molecular Devices). Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. Open the program, and set the temperature to 37 °C for preheating. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). The inhibitory rate of ingredients was calculated as follows: Inhibition (%) = (FI of control – FI of treatment) / FI of control × 100. SpectraMax M5 is a fantastic plate reader - easily my #2 behind PerkinElmer (if you can afford them). -well plate reader format. Patented Hybrid Technology delivers the sensitivity of filters with the flexibility of monochromators. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384. ameters by UV-Vis assays was done on a multimode SpectraMax M5 plate reader by using 96-well plates (Fisher Scientific). 306 plate air cal fail,low light: 316 grating motor driver faulty: 307 cuv air ref fail: 317 monitor ADC faultyThe BioTek Cytation 5 cell imaging multimode reader combines automated digital widefield microscopy with conventional multimode microplate reading. Extracting viral DNA from 4 x 109 copies of the AAV8 preparation ( nominally 5 ng ssDNA)The SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. The software is simple to use and set up protocols. reader (Molecular Devices). 7°C and the bottom to 36. edu, 814-863-3503. Devices’ StakMax® Microplate Stacker in a matter of minutes and . Dear Nelly, In a micro plate the fluorescence emission optics are very different from those in a single cell because of obstruction, reabsorption etc. Windows® 8. S. An absorbance plate reader offers higher throughput and can measure the absorbance of samples in microplates (typically 96-well or even 384-well) by sending light through each. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. Luminescence was detected using the Bright-Glo Luciferase Assay System (Promega) on a SpectraMax M5 plate reader (Molecular Devices). Phil Merta reviewed BioTek's EPOCH2 Plate Reader/Spectrophotometer and found it versatile and user-friendly. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. edu, 814-863-3503. Add to Cart. 72 MB. The name is the same, but the attitude is completely different. I have a 2002 M5 and my medium-deluxe Harbor Freight reader doesn't talk to the BMW whatsoever. Our luminescence plate readers, also referred to as luminometers are ideal for measuring a wide range of assays including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminescence resonance energy transfer. After addition of the freshly diluted substrate (50 μL/well, from Promega), bioluminescence was immediately measured on a SpectraMax M5 plate reader (Molecular Devices) using the luminescence mode. no. SpectraMax M-series Microplate readers. We use Spectramax M5 microplate reader in our lab. Quantitative PCR RNA was collected in duplicate from 1 × 10 6 cells lysed in TRIzol Reagent (Invitrogen, 15596018) after. During the room temperature incubation (step 9. 1. The cell lysate was then transferred to a white opaque 96-well plate (50 μl/well), mixed with the freshly diluted substrate (50 μl/well), and bioluminescence was immediately measured on a SpectraMax M5 plate reader (Molecular Devices) using the luminescence mode. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. 25 fmol/well, 384-well plate)−top Fluorescein 4 pM (0. The % PEG midpt and apparent solubility values (thermodynamic activity) were calculated as described previously. Web Information The compact FLx800™ fluorescence reader provides high performance in 6- to 384-well microplates at an attractive price. 2. 2. Flock Safety is an Atlanta-based company that sells automatic license plate detection readers, or ALPRs for short, to law enforcement agencies, schools, neighborhood homeowners associations and. A standard curve was plotted using various numbers of live cells and used to calculate the bacterial viability (%) in the test samples. Enter Sample IDs (HPV-Type, Sample Description and Data Reference when applicable), Dilution Factors, and background information into the template. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Copia stocks a full line of used microplate readers. Prepare a 1:1 solution of Greiss Reagent 1:Greiss Reagent 2. Tablet PC. The optics of the Gemini XPS Reader are engineered for superior performance and reliability. Bacterial growth was carried out at 25 °C in a 200 l volume withconstantshakingat450rpm. After that, DCFH-DA was used to mark cells and the ROS level was determined by Spectramax M5 plate reader and five parallel samples were prepared for each group (Wu et al. 5 mmol/L Li 2 SO 4 for 48 h. Activation of reporter constructs was monitored using the Dual-Glo Luciferase assay (Promega) and measured in a Spectramax M5 plate reader. A barcode reader accessory can be purchased with theIf you want to get the most sensitivity, you should set the plate reader excitation and emission wavelengths to different values for the two dyes. The inhibitory rate of ingredients was calculated as follows: Inhibition (%) = (FI of control – FI of treatment) / FI of control × 100. 1. SpectraMax M5 Plate Reader (Molecular Devices) If you have trouble accessing this page and need to request an alternate format, contact [email protected]. Step 1: Open the SoftMax Pro IMAP FP protocol. Thank you!. Microplate Readers. The peptide concentration was. After the plate is read, data will appear in the plate section: 11. † Cells in the paper scaffolds and collected in the well plates were also imaged with an Olympus IX-70 fluorescence microscope equipped with a 10×. Microplate Reader, Multimode. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. View the Molecular Devices website. We have run the program successfully on macOS High Sierra using the built-in Terminal and Windows 10 using Anaconda Prompt, processing data from a Molecular Devices SpectraMax M5 plate reader. Fluorescence was measured with the SpectraMax M5 plate reader with the same excitation and emission wavelengths as the chemical denaturation assay. RNA-Seq . Top reading is usually more sensitive. Each microtiter plate was sampled three times on different days, and each of the recipient plates were separately assayed with a BioTek Synergy2 microplate reader. 51 L/g). 4. To monitor the aggregation kinetics, 10 μM ThT was added to a small aliquot (300 μL) of the mixture, and the fluorescence signal (λ ex = 444 nm, λ em = 485 nm) was monitored on a Spectramax M5 plate reader (Molecular Devices). Temperature control range from Ambient +4º to 50ºC. 3 Kg) Regulatory Regulatory CE and TUV marked. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Uncontrolled Copy for Training and Reference Purposes Only . Turn the reader on and secure the USB cable. com 10. 25 For direct lysis of clots by plasmin, plasmin (250nM) was added to the surface of clots and lysis monitored by theSteady-state affinity measurements were performed on an Analyst AD, Analyst HT, or SpectraMax M5 plate reader (Molecular Devices). In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Empirical Phase. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Catalog number: 165306. Clear Filters. For the Evans blue (EB) dye extravasation test, 3,8. Select Update Driver. reader. The supernatants collected in Subheading 3. Fluorescence Microplate Readers. Activity assays were performed in clear, flat-bottomed 96-well plates and the decrease in absorbance at 340 nm was recorded using a SpectraMax M5 plate reader (Molecular Devices).